MicroFluere® Powered by Microfluidics
- Total test time under 1 hr. using pre-coated MicroFluere®
- Reduced reagent and sample volume – 20 µL vs. 100 µL
- Dynamic range >103
- MicroFluere ® plate has 96 microfluidic units arranged in the same ANSI compliant format as a traditional 96-well plate.
- Compatible with existing 96-well processing equipment and readers
- Each microfluidic unit has a liquid inlet, an optically clear detection channel containing micro-posts, and a liquid outlet. In the microfluidic unit, samples and reagents flow from a funnel-shaped structure through a micro-post array embedded microfluidic channel.
- The liquid can be withdrawn from an opening outlet using a wicking method or pressure differential. The optical signal is acquired at the center of the microfluidic loop located at the standard plate reader optical excitation/collection position. Since the inlet and outlet are offset from the detection area, there is no interference to the optical signal caused by sample/reagent residuals at the inlet (i.e., funnel-shaped structure) or the outlet.
- In the looped microfluidic channel, an array of optically-transparent micro-posts are arranged in a symmetric pattern. The micro-posts extend perpendicularly from the top of the channel. The micro-posts are equally distributed throughout the entire detection area, and each micro-post has a generally cylindrical shape. The unique arrangement of the channel and the micro-posts within each microfluidic unit assures consistent optical measurements even in the presence of as large as 1 mm positive or negative lateral shift of the plate in the horizontal (X- axis) and/or vertical (Y-axis) direction with respect to the optical detection center. The results show that CVs (coefficient of variations) due to both X and Y movements are less than 5% (note: the plate reader itself may already cause 5% variation in optical signal reading).
- 10730044, Assay Plate and Uses Thereof, 10730044 (issued in 2020)
- 62657834, Differential Pressure Assisted Drainage System (pending)
- PCT/US20/52631, Microfluidic structure for bio-assay using absorption or colorimetric methods (pending)
- 63/001,366, Methods for enzyme-linked immunosorbent assay (ELISA) using microfluidic plate (pending)
- US 2019/0383750 A1, OPTOFLUIDIC DIAGNOSTICS SYSTEM (pending)
- Two additional patent applications (to be submitted)
Our Publications in Express ELISA
- X. Tan et al., “Rapid Mouse Follicle Stimulating Hormone Quantification and Estrus Cycle Analysis Using an Automated Microfluidic Chemiluminescent ELISA System,” ACS Sens. 3, 2327-2334 (2018).
- X. Tan et al., “Multiparameter Urine Analysis for Quantitative Bladder Cancer Surveillance of Orthotopic Xenografted Mice,” Lab Chip 20, 634-646 (2020).
- W. Xue et al., “Rapid and sensitive detection of drugs of abuse in sweat by multiplexed capillary based immuno-biosensors,” Analyst 145, 1346-1354 (2020).
- X. Tan et al., “Rapid and quantitative detection of SARS-CoV-2 specific IgG for convalescent serum evaluation,” Biosensors and Bioelectronics 169, 112572 (2020)
- X. Tan et al., “Quantification and Immunoprofiling of Bladder Cancer Cell-Derived Extracellular Vesicles with Microfluidic Chemiluminescent ELISA,” (to be submitted)