Description

OptoBio’s Human IFN-gamma ELISA kit containing 5 pre-coated MicroFluere 96-well microplates allows 35-minute assay and delivers a dynamic range of 23.44 pg/ml – 12,000 pg/ml.  This kit is for Research Use Only (RUO).  The kit contains:

1. 5 MicroFluere 96-well plates coated with a mouse anti-Human Interferon Gamma (IFNG antibody, rabbit monoclonal)
2. Standard- Each vial contains 40 ng of recombinant Interferon Gamma (IFNG)
3. Detection Antibody – 0.2 mg/mL of mouse anti-Human Interferon Gamma, IFNG monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50% HRP-Protector)
4. Dilution Buffer (A) for sample and standard
5. Dilution Buffer (B) for detection antibody
6. 100 absorbent pads

The kit does not contain required QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit (Catalog number: 15159).

Reading should be with a Fluorescence microplate reader ( ~550 nm excitation and 605 nm emission wavelengths)

MicroFluere IFNg kit Spec-sheet and Getting Started with MicroFluere