MicroFluere Benefits

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MicroFluere Benefits                                                                5X-10X Faster Assay
                                                                                                     5X Smaller Reagent Volume
                                                                                                     5X Smaller Sample Volume
                                                                                                     10X Higher Dynamic Range

Comparison of ELISA Protocol

  • 10 steps rather than 21 steps in traditional ELISA for the same protocol
  • About 1.5 hrs. for the entire process
  • Dramatically reduce time for optimizing ELISA protocol

MicroFluere®

  • Use 20 µL or less
  • Fewer steps
MicroFluere® Incubation (min) Step
Capture 60 #1
Blocking 30 #2
Wash 0 #3
Standard 15 #4
Detection 15 #5
HRP 5 #6
Wash 0 #7
Wash 0 #8
Wash 0 #9
Substrate 5 #10
TOTAL 130 10
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Capture antibody coating
90 minutes

Actual assay: 40 minutes

Traditional

  • Use 100 µL or more
  • More steps
Traditional Incubation* (min) Step
Capture 540 #1
Wash 0 #2
Wash 0 #3
Wash 0 #4
Blocking 60 #5
Wash 0 #6
Wash 0 #7
Wash 0 #8
Standard 120 #9
Wash 0 #10
Wash 0 #11
Wash 0 #12
Detection 120 #13
Wash 0 #14
Wash 0 #15
Wash 0 #16
HRP 20 #17
Wash 0 #18
Wash 0 #19
Wash 0 #20
Substrate 20 #21
TOTAL 880 21
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Capture antibody coating 
Overnight + 60 minutes
Actual assay: 280 minutes

Benchmarking Test Results

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Using Human IL-6 (DY206) kit from R&D Systems

Cost Analysis

Description Traditional well plate MicroFluere® Cost saving %
R&D Systems reagents $0.44/well $0.15/well 66%
Substrate from ThermoFisher $0.32/well $0.04/well 88%
Total reagent cost $0.76/well $0.19/well 75%
Well plate $0.01/well $0.21/well -133.33%
Total $0.77/well $0.40/well 53%
Cost assumption
Human IL-6 Duo kit for 5 traditional well plate 1 Kit $200.00
Reagent diluent 21 ml/vial $40.00
Washing 100 ml/vial $40.00
PBS 250 ml/vial $55.00
Substrate (Fluorescence) 110 ml/pk $350.00
Traditional 96-well plate 1 plate $5.00
MicroFluere® 1 plate $20.00

Retail cost of Human IL-6 ELISA kit with absorbance substrate: $519/plate (capture antibody pre-coated plate)
Cost of our own kit using traditional 96-well plate with fluorescence substrate: $73.92/plate (see table above)
Cost of our own kit using MicroFluere® well plate with fluorescence substrate: $38.40/plate (see table above)

Value Proposition for COVID-19

MicroFluere® plate Traditional plate
Reagent volume 20 µL 100 µL
Coating time 1 hour 24 hours
Assay time for antibody 20 minutes >3-4 hours
Detection limit for antibody Same (can be further optimized) 5 ng/mL for antibody
Assay time for cytokine 40 minutes >6 hours
Detection limit for cytokine A few pg/mL Same
Dynamic range 10X higher
Number of samples per plate 80 80
Internal standards Yes (16) Yes (16)

Reagent Cost of COVID-19 tests (Large-scale assay -- MicroFluere®) S Protein

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Reagent Cost of COVID-19 tests(Large-scale assay -- MicroFluere®)N Protein

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Reagent Cost of COVID-19 tests(Large-scale assay -- MicroFluere®)IgG Antibody

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